Identification and characterization of resistance to cowpea aphid (Aphis craccivora Koch) in Medicago truncatula
نویسندگان
چکیده
منابع مشابه
Characterization of pea aphid resistance in Medicago truncatula.
To achieve a thorough understanding of plant-aphid interactions, it is necessary to investigate in detail both the plant and insect side of the interaction. The pea aphid (PA; Acyrthosiphon pisum) has been selected by an international consortium as the model species for genetics and genomics studies, and the model legume Medicago truncatula is a host of this aphid. In this study, we identified ...
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Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is required. In this study, ten candidate HKGs including elongation factor 1 α (EF1A), ribosomal protein L11 (RPL11), ribosomal protein L14 (RPL14), r...
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The performance of cowpea aphid, Aphis craccivora Koch. (Hemiptera: Aphididae), on five faba bean, Vicia faba L. (Fabales: Fabaceae) cultivars was evaluated. Colony development, biology, and demographic parameters were studied to measure the cowpea aphid performance. Two methods, whole plant and detached leaf, were used in these experiments. After 14 d , the number of apterous adult, nymphs, an...
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Aphids cause significant yield losses in agricultural crops worldwide. Medicago truncatula, a model legume, cultivated pasture species in Australia and close relative of alfalfa (Medicago sativa), was used to study the defence response against Therioaphis trifolii f. maculate [spotted alfalfa aphid (SAA)]. Aphid performance and plant damage were compared among three accessions. A20 is highly su...
متن کاملTemperature and Development Impacts on Housekeeping Gene Expression in Cowpea Aphid, <em>Aphis craccivora</em> (Hemiptera: Aphidiae)
Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is required. In this study, ten candidate HKGs including elongation factor 1 α (EF1A), ribosomal protein L11(RPL11), ribosomal protein L14(RPL14), rib...
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ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2012
ISSN: 1471-2229
DOI: 10.1186/1471-2229-12-101